Efficient production of recombinant PP2A at a low temperature using a baculovirus expression system
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Efficient production of recombinant PP2A at a low temperature using a baculovirus expression system
Protein phosphatase 2A (PP2A) is an enzyme useful for detecting several natural toxins represented by okadaic acid and microcystins. We found that the production of the recombinant human PP2A catalytic subunit (rhPP2Ac) in High Five insect cells could markedly increase when the cells were cultured at 19 °C instead of 27 °C used under conventional conditions. The yield and purity of the enzyme i...
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ژورنال
عنوان ژورنال: Biotechnology Reports
سال: 2016
ISSN: 2215-017X
DOI: 10.1016/j.btre.2016.07.004